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2385s  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc 2385s
    2385s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2385s/Phospho-IRS-1+(Ser1101)+Antibody/pmc12925166-9-7-9
    Average 95 stars, based on 144 article reviews
    2385s - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: CXCR1/2 Inhibitor Ladarixin Ameliorates the Insulin Resistance of 3T3-L1 Adipocytes by Inhibiting Inflammation and Improving Insulin Signaling
    Article Snippet: Non-specific binding sites were blocked by Blocking Buffer (Invitrogen, USA) for 10 min at RT. .. Membranes were then incubated overnight at 4 °C with the following primary antibodies, diluted in blocking buffer: mouse anti-tubulin alpha 1:2000 (ab80779, lot: GR209729-4, Abcam, Cambrige, UK); mouse anti-AP2 1:200 (MA1-872, lot:UL296310, Invitrogen, USA), rabbit anti-PPARγ 1:1000 (ab209350, lot:GR3284934-2, Abcam, Cambridge, UK), rabbit anti-Pref1 1:200 (MBS9210091, LOT: SA141106AX, MyBioSource, San Diego, CA, USA), rabbit anti-GLUT4 1:1000 (PA5-23052, lot: PH1895639, Thermo, USA), rabbit anti-NfkB 1:1000 (ab16502, lot:GR3309481-1, Abcam, USA), rabbit anti-p-IRS1 1:500 (2385S, lot:2, Cell Signaling, Danvers, MA, USA), rabbit anti-p-IRS2 1:500 (4502S, lot:6, Cell Signaling, USA), rabbit anti-IRS 1:500 (3407S, lot:6, Cell Signaling, USA) rabbit anti-PI3k 1:1000 (ab151549, lot:GR3235734, Abcam, USA), rabbit anti-p-AKT (ab81283, lot:GR240003, Abcam, USA) and rabbit anti-AKT 1:1000 (9272S, lot:2, Cell Signaling, USA), mouse conjugated Actin 1:20000 (5125S, lot:6, Cell Signaling, USA). .. As secondary antibodies, 1:20,000 peroxidase-conjugated anti-rabbit (111-035-003, lot:149393, Jackson Immuno Research, Ely, UK) or anti-mouse IgG (115-035-003, lot:149723, Jackson Immuno Research, Cambridge, UK) were used.

    Blocking Assay:

    Article Title: CXCR1/2 Inhibitor Ladarixin Ameliorates the Insulin Resistance of 3T3-L1 Adipocytes by Inhibiting Inflammation and Improving Insulin Signaling
    Article Snippet: Non-specific binding sites were blocked by Blocking Buffer (Invitrogen, USA) for 10 min at RT. .. Membranes were then incubated overnight at 4 °C with the following primary antibodies, diluted in blocking buffer: mouse anti-tubulin alpha 1:2000 (ab80779, lot: GR209729-4, Abcam, Cambrige, UK); mouse anti-AP2 1:200 (MA1-872, lot:UL296310, Invitrogen, USA), rabbit anti-PPARγ 1:1000 (ab209350, lot:GR3284934-2, Abcam, Cambridge, UK), rabbit anti-Pref1 1:200 (MBS9210091, LOT: SA141106AX, MyBioSource, San Diego, CA, USA), rabbit anti-GLUT4 1:1000 (PA5-23052, lot: PH1895639, Thermo, USA), rabbit anti-NfkB 1:1000 (ab16502, lot:GR3309481-1, Abcam, USA), rabbit anti-p-IRS1 1:500 (2385S, lot:2, Cell Signaling, Danvers, MA, USA), rabbit anti-p-IRS2 1:500 (4502S, lot:6, Cell Signaling, USA), rabbit anti-IRS 1:500 (3407S, lot:6, Cell Signaling, USA) rabbit anti-PI3k 1:1000 (ab151549, lot:GR3235734, Abcam, USA), rabbit anti-p-AKT (ab81283, lot:GR240003, Abcam, USA) and rabbit anti-AKT 1:1000 (9272S, lot:2, Cell Signaling, USA), mouse conjugated Actin 1:20000 (5125S, lot:6, Cell Signaling, USA). .. As secondary antibodies, 1:20,000 peroxidase-conjugated anti-rabbit (111-035-003, lot:149393, Jackson Immuno Research, Ely, UK) or anti-mouse IgG (115-035-003, lot:149723, Jackson Immuno Research, Cambridge, UK) were used.

    Western Blot:

    Article Title: PIM Kinases Alter Mitochondrial Dynamics and Chemosensitivity in Lung Cancer
    Article Snippet: JC-1, mitoSOX-red and H 2 -DCFDA were purchased from Invitrogen. .. Primary antibodies for immunoblot and immunofluorescence analyses were purchased from BD Biosciences [Actin, 612656], Santa Cruz [Drp1, sc-271583, PIM1, sc-13513 and Bcl-2, sc-7382], and Cell Signaling Technology [TOM20, 4240S; pIRS (S1101), 2385S; PIM1, 3247S; pDRP1 (S616), 3455S; pDRP1 (S637), 4867S; and cleaved caspase-3, 9661S. ..

    Article Title: PIM Kinases Alter Mitochondrial Dynamics and Chemosensitivity in Lung Cancer
    Article Snippet: JC-1, mitoSOX-red and H 2 -DCFDA were purchased from Invitrogen. .. Primary antibodies for immunoblot and immunofluorescence analyses were purchased from BD Biosciences [Actin, 612656], Santa Cruz [Drp1, sc-271583, PIM1, sc-13513 and Bcl-2, sc-7382], and Cell Signaling Technology [TOM20, 4240S; pIRS (S1101), 2385S; PIM1, 3247S; pDRP1 (S616), 3455S; pDRP1 (S637), 4867S; and cleaved caspase-3, 9661S. ..

    Immunofluorescence:

    Article Title: PIM Kinases Alter Mitochondrial Dynamics and Chemosensitivity in Lung Cancer
    Article Snippet: JC-1, mitoSOX-red and H 2 -DCFDA were purchased from Invitrogen. .. Primary antibodies for immunoblot and immunofluorescence analyses were purchased from BD Biosciences [Actin, 612656], Santa Cruz [Drp1, sc-271583, PIM1, sc-13513 and Bcl-2, sc-7382], and Cell Signaling Technology [TOM20, 4240S; pIRS (S1101), 2385S; PIM1, 3247S; pDRP1 (S616), 3455S; pDRP1 (S637), 4867S; and cleaved caspase-3, 9661S. ..

    Article Title: PIM Kinases Alter Mitochondrial Dynamics and Chemosensitivity in Lung Cancer
    Article Snippet: JC-1, mitoSOX-red and H 2 -DCFDA were purchased from Invitrogen. .. Primary antibodies for immunoblot and immunofluorescence analyses were purchased from BD Biosciences [Actin, 612656], Santa Cruz [Drp1, sc-271583, PIM1, sc-13513 and Bcl-2, sc-7382], and Cell Signaling Technology [TOM20, 4240S; pIRS (S1101), 2385S; PIM1, 3247S; pDRP1 (S616), 3455S; pDRP1 (S637), 4867S; and cleaved caspase-3, 9661S. ..



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